اثر شیلاجیت بر سطح سایتوکاین های پیش التهابی و ضد التهابی در آسیب کبدی در موش صحرایی نر

Background and purpose: Cytokines regulate&nbsp;a broad range of&nbsp;biological and pathological processes. They play an important role in liver damage. Inflammatory and antioxidant factors are important causes of liver damage. Shilajit is known as an anti-inflammatory and antioxidant agent. Therefore, the main aim of this study was to determine the effect of Shilajit on levels of proinflammatory and anti-inflammatory cytokines in liver injury. Materials and methods: In this experimental study, 80 male Wistar rats were randomly divided into two main groups: A and B. Each group was organized into five subgroups: control, acetaminophen (A) or bile duct obstruction (BDL) (B), low dose, high dose, and vehicle. All subgroups except the controls in group A received 500 mg/ kg acetaminophen via IP injection, while BDL was performed in all subgroups in group B. Then in group A, two hours and&nbsp; in group B seven days after injury, the treatment subgroups with drug and vehicle received 150 mg/kg (low dose) and 250 mg/kg (high dose)shilajit&nbsp; and distilled water as vehicle, for one and six days, respectively. After completing treatment, liver samples were taken for laboratory analysis. Results: Low-dose Shilajit treatment reduced IL-10, IL-6, IL-1&beta;, and TNF- &alpha; levels, which had been increased by a single dose of 500 mg/kg acetaminophen (p<0.05). Conclusion: Shilajit is effective in reducing inflammation and liver damage caused by high-dose consumption of acetaminophen. &nbsp;